A New Era of Cell    Screening Sciences

Only the combination of these key features ensures the potential for fully flexible

assay development and the industrial screening process. Key for high resolution is

strictly confocal imaging and the use of water immersion lenses. Main driver for

speed is simultaneous image acquisition and data processing. The most prominent

areas of use are cell screening at sub-cellular resolution level as well as bead based

screening applications - from conventional plate formats to nanoplate scale.

Features

Fully automated system

True point confocal

Multiple high power lasers

Simultaneous multi color imaging

Variable magnification for the

resolution of choice

Highly flexible choice of sample carrier

Highest data acquisition/processing rates:

>100,000 multi color data points in 24h

Optional: Fluorescence lifetime imaging

system

Optional: On board dispensing

Off-the-shelf as well as custom

designed hardware solutions according

to customer’s needs

Comprehensive and fully flexible

image analysis software Libretto as

on-board solution

Applications

Whole cell fluorescence assays

Cell viability, cell differentiation, cell

proliferation, cytotoxicity, apoptosis,

transporter phenomena

Cell signaling assays

Calcium flux, second messengers, ion

channels/membrane potential

Gene expression assays

Expression of house-keeping as well

as reporter genes, gene acitivity and

protein regulation, RNAi

Membrane receptor assays

Ligand binding, receptor activation

and desensitization, translocation and

endocytosis recruitment of signaling

molecules

Translocation assays

Target molecule redistribution

Morphological assays

Neurite outgrowth, cell differentiation,

cell adhesion and spreading

Immunochemical assays

Bead-based homogeneous antigen-antibody interaction

Disclaimer: For the use of the assays described

additional licenses may be required.

Cells are identified using a broadrange  dye.

Single cell results (above) and EC50

curves for two activating compounds (below).say prep

Run assay in appropriate 96-, 384- or 1536-well micro titer plates.

Transfer assay plate to the Opera (manually or by any robotic system) and apply appropriate mode of reading/dataacquisition (e.g. single or multi image well reading, z-scans, time lapse).Apply on-board device-inherent or user-defined scripts and analyze data on-line during image acquisition or re-analyze data from a remote PC. Store raw and processed data in a file based system.